In the other EXSY experiment, the EXSY reference experiment, no cross peaks due to magnetization exchange should be observed (thermal equilibrium) and the amplitudes of just the diagonal peaks of those signals in exchange, A(0), have to be measured. In this experiment the amplitudes (intensities) of those signals in exchange, A(tm), have to be quantified for both diagonal and cross peaks.
In the former experiment the mixing time (tm) need to be large enough for the magnetization exchange process to take place. For the calculation of rate constants, the program requires that the user supplies the experimental amplitudes of certain NMR peaks obtained in two different EXSY experiments, one is an EXSY experiment acquired at a certain mixing time (tm), and the other is an EXSY experiment acquired at 0 or very short mixing time (reference experiment).
